RESUMO
No disponible
Assuntos
Humanos , Masculino , Pessoa de Meia-Idade , Mucormicose/microbiologia , Cunninghamella/patogenicidade , Transplante de Rim , Insuficiência Respiratória/etiologia , Hospedeiro Imunocomprometido , Vírus da Influenza A Subtipo H1N1/patogenicidade , Influenza Humana/complicaçõesRESUMO
No disponible
Assuntos
Humanos , Feminino , Pessoa de Meia-Idade , Ceratite/tratamento farmacológico , Nocardia , Ceratócitos da Córnea , Ceratócitos da Córnea/patologia , Soluções Oftálmicas/administração & dosagem , Lubrificantes Oftálmicos/administração & dosagem , Gentamicinas/administração & dosagem , Ceratite/diagnóstico , Nocardia/isolamento & purificação , Ácido Fusídico/administração & dosagem , Cloranfenicol/administração & dosagem , Tobramicina/administração & dosagem , Corticosteroides/administração & dosagemRESUMO
Introducción: Un porcentaje variable de muestras analizadas por el equipo cobas 4800 pueden dar un resultado invalidado por inhibición de la PCR o erróneo al no extraerse el ADN correctamente con el test cobas 4800 CT/NG. Método: Valoración de un protocolo de agitación y dilución de la muestra original (exudado u orina) en un total de 116 muestras. Para analizar la sensibilidad de este método, 100 muestras (exudados y orinas) con resultado conocido fueron retestadas. Resultados: Un 98,3% (114/116) de las muestras se resolvieron con este protocolo con un 100% de concordancia al consultar con datos clínicos, tinción de Gram y otras muestras analizadas en paralelo del mismo paciente. Discusión: Los datos indican que no hay pérdida de sensibilidad con este protocolo, por lo que los usuarios de esta plataforma podrían usarlo sin necesidad de métodos alternativos (AU)
Introduction: A variable percentage of samples analysed using the Cobas 4800 assay can give an invalid result by PCR inhibition or erroneous due to incorrect DNA extraction with the Cobas 4800 CT/NG test. Method: An analysis was performed using the vortex agitation and dilution protocol on the original sample (swab or urine) for a total of 116 samples. In order to analyse the sensitivity of this method, 100 samples (swabs and urine) with known results were retested. Results: A total of 98.3% (114/116) of the samples analysed were resolved with this protocol with 100% agreement after reviewing clinical data, Gram stain, and other samples analysed in parallel from the same patient. Discussion: The data indicate no loss of sensitivity with this protocol; thus Cobas 4800 users could use this method without the need for alternative methods (AU)
Assuntos
Humanos , Chlamydia trachomatis/isolamento & purificação , Infecções por Chlamydia/microbiologia , Neisseria gonorrhoeae/isolamento & purificação , Gonorreia/microbiologia , Infecções Sexualmente Transmissíveis/microbiologia , Técnicas de Diluição do Indicador , Reação em Cadeia da Polimerase/métodos , Erros de Diagnóstico/prevenção & controleRESUMO
Testing for Candida albicans germ-tube antibody IFA IgG assay (CAGTA) is used to detect invasive candidiasis infection. However, most suitable assays lack automation and rapid single-sample testing. The CAGTA assay was adapted in an automatic monotest system (invasive candidiasis [CAGTA] VirClia® IgG monotest (VirClia®), a chemiluminescence assay with ready-to-use reagents that provides a rapid objective result. CAGTA assay was compared with the monotest automatic VirClia® assay in order to establish the diagnostic reliability, accuracy, and usefulness of this method. A prospective study with 361 samples from 179 non-neutropenic critically ill adults patients was conducted, including 21 patients with candidemia, 18 with intra-abdominal candidiasis, 84 with Candida spp. colonization, and 56 with culture-negative samples, as well as samples from ten healthy subjects. Overall agreement between the two assays (CAGTA and VirCLIA) was 85.3%. These assays were compared with the gold-standard method to determine the sensitivity, specificity as well as positive and negative predictive values. In patients with candidemia, values for CAGTA and VirCLIA assays were 76.2 versus 85.7%, 80.3 versus 75.8%, 55.2 versus 52.9%, and 91.4 versus 94.3%, respectively. The corresponding values in patients with intra-abdominal candidiasis were 61.1 versus 66.7%, 80.3 versus 75.8%, 45.8 versus 42.9%, and 88.3 versus 89.3%, respectively. No differences were found according to the species of Candida isolated in culture, except for Candida albicans and C. parapsilosis, for which VirClia® was better than CAGTA. According to these results, the automated VirClia® assay was a reliable, rapid, and very easy to perform technique as tool for the diagnosis invasive candidiasis.
Assuntos
Anticorpos Antifúngicos/sangue , Automação Laboratorial/métodos , Candida albicans/imunologia , Candidíase Invasiva/diagnóstico , Imunoensaio/métodos , Testes Sorológicos/métodos , Humanos , Imunoglobulina G/sangue , Unidades de Terapia Intensiva , Medições Luminescentes , Valor Preditivo dos Testes , Estudos Prospectivos , Sensibilidade e EspecificidadeRESUMO
INTRODUCTION: A variable percentage of samples analysed using the Cobas 4800 assay can give an invalid result by PCR inhibition or erroneous due to incorrect DNA extraction with the Cobas 4800 CT/NG test. METHOD: An analysis was performed using the vortex agitation and dilution protocol on the original sample (swab or urine) for a total of 116 samples. In order to analyse the sensitivity of this method, 100 samples (swabs and urine) with known results were retested. RESULTS: A total of 98.3% (114/116) of the samples analysed were resolved with this protocol with 100% agreement after reviewing clinical data, Gram stain, and other samples analysed in parallel from the same patient. DISCUSSION: The data indicate no loss of sensitivity with this protocol; thus Cobas 4800 users could use this method without the need for alternative methods.
Assuntos
Técnicas de Tipagem Bacteriana/instrumentação , Chlamydia trachomatis/isolamento & purificação , DNA Bacteriano/análise , Neisseria gonorrhoeae/isolamento & purificação , Reação em Cadeia da Polimerase em Tempo Real/instrumentação , Manejo de Espécimes/métodos , Técnicas de Tipagem Bacteriana/métodos , Portador Sadio/microbiologia , Colo do Útero/microbiologia , Infecções por Chlamydia/microbiologia , Chlamydia trachomatis/genética , DNA Bacteriano/isolamento & purificação , Exsudatos e Transudatos/microbiologia , Feminino , Gonorreia/microbiologia , Humanos , Masculino , Neisseria gonorrhoeae/genética , Reação em Cadeia da Polimerase em Tempo Real/métodos , Reto/microbiologia , Sensibilidade e Especificidade , Urina/microbiologiaRESUMO
PCR methods are nowadays between the most rapid and sensitive methods for screening and diagnosing herpes simplex virus (HSV) type 1 and 2. The aim of this study was to analyze the reliability, accuracy, and usefulness of the new assay HSV OligoGen kit in comparison with the Roche LightCycler HSV ½ Qual Kit assay for the detection of HSV in clinical samples. For this analysis, a prospective study was designed for detection of HSV-1 and HSV-2 including 110 ulcer specimens, 48 urine, 48 endocervical, 43 cerebral spinal fluids, 4 urethral and 3 pharyngeal swabs that were sent from a regional STI clinic or an Intensive Clinical Unit, both in Seville, Spain. In comparison to the Roche LightCycler HSV ½ Qual Kit assay, sensitivity, specificity, positive and negative predicative values, and kappa value for HSV detection using the HSV OligoGen kit were 96.2%, 100%, 100%, 98.3%, and 0.97 for HSV-1, respectively. For HSV-2, the corresponding values were 98.3%, 100%, 100%, 99.5%, and 0.98, respectively. Statistical data obtained in this study confirms the usefulness and reliable results of this new assay.
